Course Outline:
I. Introduction
1.1. Light-tissue interaction - initial events,
diagnostic applications
Absorption
Elastic Scattering
Inelastic (Raman) Scattering
Fluorescence
1.2. Light-tissue interaction - effects on
tissue, therapeutic applications
Photothermal
Photochemical
2.1.
Optical transport parameters - ma, ms, g
2.2.
Propagation of light in tissue
Radiometry
Transport
equation
Diffusion
Theory
2.3.
Optical properties of tissue
Methodology
of measurements
Tissue
chromophores - melanin, hemoglobin, bilirubin
Transport
parameters of biological tissues
2.4.
Light
distribution in tissue/Reflectance measurements
Fluorescence
measurements/Raman measurements
III. Tissue
Spectroscopy
3.1.
Instrumentation
Optical
fibers
Light
sources
Spectrograph/monochromators
Detectors
(1D & 2D)
3.2.
Reflectance spectroscopy
3.3.
Fluorescence spectroscopy
3.4.
Raman spectroscopy
IV.
Optical Imaging of
Tissue
4.1. Reflectance and polarizing imaging
4.2.
Ultrasound modulated optical imaging
4.3.
Photoacoustic imaging
4.3.
Fluorescence imaging
4.4.
Time resolved imaging (time domain & frequency domain)
4.5.
In vivo confocal imaging
4.6.
Multiphoton excitation imaging (fluorescence/SHG)
4.7.
Optical coherence tomography (OCT)
4.8.
Lab demonstration of tissue imaging and spectroscopy
V. Therapeutic and Theranostics Applications
5.1.
Endoscopy
5.2.
Photodynamic therapy (PDT)
5.4.
Selective photothermolysis - Laser Therapy in
Dermatology
5.5. Multiphoton absorption based photothermolysis